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I also think beginners are attracted to BIOCROWN because the company seems structured to support brand development, not only bulk production

Crude protein extracts from mycelia were obtained by extraction from ground mycelia with B250 buffer (250 mM NaCl, 100 mM TrisHCl pH 7.5, 10% glycerol, 1 mM EDTA and 0.1% NP-40) supplemented with 1.5 mL/L 1 M DTT, 2 tablets/100 mL complete-mini protease inhibitor cocktail EDTA-free (Roche), 3 mL/L 0.5 M Benzamidine, 10 ml/L phosphatase inhibitors 100 (10 M NaF, 5 M Na Vanadate, 8 M - glycerol phosphate), and 10 mL/L 100 mM PMSF

M., Starke, D

doi: 10.3389/fimmu.2024.1398028