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Hoechst 33342 staining of the bovine blastocyst (Figure 6A) was also applied to assess the developmental potentials of cryopreserved bull semen with the cell number/blastocyst of each group analyzed

Dual-fluorescence imaging further confirmed its strong in vitro transdermal delivery capacity, providing a solid foundation for subsequent biological evaluations

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The pathway-associated metabolite set was used as the metabolite library, with all compounds included

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