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Description
Sirt3 blocks the cardiac hypertrophic response by augmenting foxo3a-dependent antioxidant defense mechanisms in mice

the freeze and subsequent thaw step provides a more uniform product by removing granulocytes and to some extent monocytes in the cell population

10.1007/s00425-005-0006-1 147 Ruttkay-NedeckyB.NejdlL.GumulecJ.ZitkaO.MasarikM.EckschlagerT.et al

Mix 1 vial of Vitamin C, 1 ampoule of Cindella, and 1 vial of dissolved Luthione to the 50ml PNSS

More cell biology would seem to be in order to address these issues
