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Compatibility with downstream applications Sensitivity and specificity of the labeling method Impact on nucleic acid function Stability and photostability of the fluorescent label Cost and ease of use Limitations include potential interference with nucleic acid function, background fluorescence, photobleaching (loss of fluorescence over time), and the need for optimization based on specific experimental requirements.

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[DOI] [PubMed] [Google Scholar] Pires JM, Foresti ML, Silva CS, Rgo DB, Cali ML, Mosini AC, Nakamura TKE, Leslie ATF, Mello LE

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