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doi: 10.1111/J.1530-0277.2009.01031.X
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Mark each vial clearly with the date of first puncture and the 28-day discard date using permanent marker
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The cell cultures were maintained in RPMI-1640 media and supplemented with 10% FBS and 1% penicillin/streptomycin at 5% CO2 in an incubator
