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Description
FOXO4 facilitates TM3 Leydig cell senescence and maintains the viability of senescent cells To further validate the role of FOXO4 in senescent Leydig cells, we silenced FoxO4 expression using siRNA prior to senescence induction
Kompletter Guide
Following 3 10min washes with 1ml 0,2% TBS-Gelatin and 1 wash with regular TBS the slides were mounted using soft set mounting medium with DAPI (Vectashield) and sealed with nail polish
The mutants were expressed using the pET system and purified using the same method as that described for wild-type DAP BII above
5. Anti-Aging & Longevity
