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After 1 h at 20 C worms were picked washed and anesthetized in a drop of levamisole and images were captured immediately using a Zeiss AxioZoom v16 microscope equipped for fluorescence illumination
The BMP signaling pathway can be categorized into either canonical or non-canonical pathways
The sequencing is personalized not a fixed package
Before imaging, the growth medium was removed, then the cells were washed with PBS (2) and imaged in FluoroBrite DMEM
