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The day after, neurospheres were washed twice with PBS 1X and further incubated for 1 h with Dy-Light -conjugated secondary antibodies (1:500, all from Abcam), and DAPI (1 g/mL, ThermoFisher) in blocking solution (3.5% BSA in PBS 1X) under constant gyratory shaking

Options include fortified plant-based milks, breakfast cereals, and nutritional yeast that contains added B12

doi: 10.1046/j.1365-2230.1998.00310.x

Glutathione injections bypass the digestive system, allowing for more efficient absorption and faster effects compared to oral supplements, which often have lower bioavailability