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Retrieved 17 April 2025

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Both strains were stored at 80C in Brucella Broth (BB, Thermo Fisher Scientific Remel Products, Lenexa, KS, United States) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Gibco, Life Technologies, Carlsbad, CA, United States) and 20% (v/v) glycerol