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Slides were treated with H2O2 (Gifrer) to block endogenous peroxidase activity, then saturated with non-immune donkey serum 2%, BSA 1%, diluted in TBS tween 0.05% pH 7.4

Altering the PUFA composition of dopaminergic neuronal membranes and co-treating with iron can synergistically amplify lipid peroxidation and trigger ferroptosis, which has been confirmed in LUHMES cell and mouse organotypic brain slice experiments (70)

In addition, other modalities, including DNA methylation, non-coding RNAs, and histone modifications, constitute another important mechanism involved in cancer development through regulation of gene expression (Wen et al., 2023, Xu et al., 2022)

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