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Shao S, Wang G, Maverakis E, Gudjonsson JE (2020) Targeted treatment for erythrodermic psoriasis: rationale and recent advances
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The supernatant was then analyzed by reverse-phase HPLC (Agilent 1260 Infinity LC, USA) on a Welch XB-C18 column (5 m, 250 mm 4.6 mm) equipped with a fluorescence detector at the excitation and emission wavelengths of 365 and 440 nm, respectively [9]

After attaining the required result use twice a week

Unfortunately, some patients fail to respond to therapy and many suffer from serious side effects, highlighting the need to investigate other agents to treat this disease