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Cell Death Dis 11:922 Feng Q, Yu X, Qiao Y, Pan S, Wang R, Zheng B et al

It comes as a freeze-dried powder, called a lyophilized powder, that a lab dissolves before any study

Simultaneously, the mechanical properties of the ECM change significantly: the shear stiffness of the human nucleus pulposus increases from 12.5 kPa in Pfirrmann grade 116.5 kPa in grade V (Walter et al., 2017), collagen fibers thicken and become disorganized (Leung et al., 2014), its aggrecan synthesis decreases while degradation increases (Wuertz et al., 2007) (Table 1)

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The sciatic nerve runs under (and in some people through) the piriformis muscle on its way out of the pelvis