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The periplasmic solution containing soluble Pmab1 Fv-clasps was clarified over a 0.45 m filter and purified by Ni 2+ affinity chromatography as follows

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[DOI] [PubMed] [Google Scholar] 719.Holtmannspotter M, Peters N, Opherk C, Martin D, Herzog J, Bruckmann H, Samann P, Gschwendtner A, Dichgans M