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To establish if the binding of Ran to PlexinB1 is dependent on whether Ran is bound to GTP or GDP, pull-down experiments were performed using purified Ran-His protein labelled with GTPS (a non-hydrolysable form of GTP) or GDP

This implies that mutation induction in directly exposed cells is regarded as the cause of this risk for humans

Subsequently, the membranes were washed thrice with TBST and the membranes were incubated with horseradish-peroxidase conjugated secondary antibodies rabbit/mouse polyclonal antibody (1:1000, Yeason) for 60 min
