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Loading: 1.0mmol/g) and pour it into the reaction column, add about 10ml of DMF, soak for 30 minutes (at room temperature) and dry it with a vacuum pump, add Fmoc-Val-OH 3.4g, DIC0.7ml, DMAP0.1g, DMF10ml, react at room temperature for 2 hours, then add blocking reagent benzoyl chloride 0.6ml, pyridine 0.4ml, DCM10ml, react at room temperature for 0.5 hours, wash the resin with isopropanol 3 times, wash with DMF 3 times, washed 2 times with DMF re-distilled, drained to obtain Fmoc-Val-WangResin

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1 mM / l MgCl