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This involves measuring reduced glutathione (GSH) and total glutathione (tGSH, comprising both free reduced GSH and newly formed reduced GSH generated from oxidised glutathione (GSSG) through reduction with tris(2-carboxyethyl)phosphine [TCEP]) in human CSF (Fig

In this process, 5,10-methylene THF play a critical role in methionine regeneration and methyl donation, meanwhile MTHFR catalyzes the irreversible conversion of 5,10-methylene THF to 5-methyl THF that participate in generation of SAM in methionine cycle and offer methyl group 23

PMID 35788126

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