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Despite these limitations, our findings underscore the feasibility and practicality of using routinely stored clinical samples for metabolomic analysis in the context of disease diagnosis, providing a valuable approach for real-world applications

Diet studies Control diet AIN 93G (17.6% protein, 7.1% fat and 11% sugar) and ketogenic diet TD.130659 (15% protein, 72% fat and 2.4% sugar) were purchased from Ssniff

The mixture of methanol and water (70:30, volume/volume) was used as the mobile phase at room temperature, the flow rate was 0.8 mL/min, the injection volume was 10 L, and the absorption wavelength was set at 254 nm